Methyl orsellinate is utilized in biochemical research as an intermediate for studying the formation of phenolic compounds in fungi and lichens. It helps identify biosynthetic pathways of lichen metabolites and is commonly used in laboratories to analyze enzyme activities involved in the methylation of orsellinic acid. It shows potential in the preliminary development of antimicrobial agents and antioxidants, exhibiting inhibitory effects on the growth of certain bacteria and the ability to reduce oxidative stress in cells. Additionally, it is studied for its role in lichen secondary metabolism, supporting ecological and biochemical investigations, with interest in pharmaceutical applications due to its structural features akin to bioactive compounds. Industrial applications are limited, primarily to laboratory and experimental uses.