Used as a crosslinking agent in molecular biology for modifying proteins and nucleic acids. It reacts selectively with thiol groups (-SH) in cysteine residues, enabling the formation of covalent crosslinks between biomolecules. Commonly applied in protein footprinting, enzyme active site labeling, and stabilizing protein-protein interactions. Also utilized in bioconjugation techniques for attaching labels or immobilizing biomolecules on surfaces. Its bifunctional iodoacetamide groups ensure specific and stable alkylation, making it valuable in structural studies and biochemical assays.