Used as a stable isotope-labeled internal standard in analytical chemistry for the accurate quantification of aflatoxin B2 in food, feed, and biological samples. Its primary application is in mass spectrometry-based methods, where it helps correct for matrix effects and instrument variability, ensuring precise and reliable detection of aflatoxin contamination. Commonly employed in food safety testing, especially in monitoring crops like peanuts, corn, and tree nuts, where aflatoxin exposure poses a health risk. Also used in metabolic studies to track the fate of aflatoxin B2 in biological systems without altering the chemical behavior due to isotopic labeling.