Calcium levofolinate is widely used in medical treatments, particularly in oncology. It serves as an antidote to counteract the toxic effects of methotrexate, a chemotherapy drug, by helping to rescue normal cells from damage. This compound is also employed in combination with fluorouracil (5-FU) to enhance its efficacy in treating colorectal cancer. By acting as a folate analog, it aids in DNA synthesis and repair, making it a critical component in cancer therapy regimens. Additionally, it is utilized in the management of folate deficiency and in certain cases of rheumatoid arthritis to support cellular function and reduce inflammation.