UV-VIS Protease Activity Measurement (Substrate: Casein)
- Product Code: 125453
UV-VIS Protease Activity Measurement (Substrate: Casein)
description ภาพรวมบริการ
UV-VIS Protease Activity Measurement (Substrate: Casein)
Anson and Folin & Ciocalteu method and Horikoshi method
PRINCIPLE
The appearance of peptides is measured as tyrosine equivalent at 660 nm by spectrophotometry.
timeline ขั้นตอนการให้บริการ
| ขั้นตอน | ขั้นตอน | ผลลัพธ์ที่คาดหวัง |
|---|---|---|
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1
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Prepare 0.1... |
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A clear 0.1 N NaOH solution.
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2
|
Prepare 1... |
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A clear 1 N NaOH solution.
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3
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Prepare 0.1... |
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A clear 0.1 M Borax solution.
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4
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Prepare 0.6%... |
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A stable, 0.6% casein solution at pH 11.0.
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5
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Prepare TCA... |
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A clear TCA mixture solution.
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6
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Prepare 0.5... |
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A clear 0.5 M Na2CO3 solution.
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7
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Prepare 1... |
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A ready-to-use Folin�s phenol reagent at 1 N concentration.
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8
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Add 3.0... |
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Test mixture containing casein and enzyme ready for reaction.
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9
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Add 3.2... |
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Reaction stopped with visible precipitate.
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10
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Centrifuge for... |
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Clear supernatant obtained for further analysis.
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11
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Transfer 2... |
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Test sample supernatant ready for color development.
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12
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Add exactly... |
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Slightly alkaline solution ready for Folin�s reagent reaction.
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13
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Immediately add... |
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Formation of a blue-colored complex with peptides containing tyrosine.
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14
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Incubate this... |
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A stable blue-colored solution proportional to proteolytic activity.
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15
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Prepare a... |
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A blank solution for absorbance correction.
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16
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After incubation,... |
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Absorbance readings at 660 nm for test and blank samples.
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17
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Prepare a... |
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Standard curve generated for protease enzyme activity determination.
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18
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Use the... |
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Enzyme activity data recorded in units/mL.
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19
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Record the... |
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Absorbance readings of test and blank samples recorded.
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20
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Subtract the... |
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Net absorbance calculated for the test sample.
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21
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Use the... |
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Final enzyme activity reported in units/mL.
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คุณจะได้รับรายงานสำหรับ Enzyme Activity , Absorbance at 660 nm ฟิลด์เมื่อเราให้บริการนี้
UV-VIS Protease Activity Measurement (Substrate: Casein)
UV-VIS Protease Activity Measurement (Substrate: Casein)
UV-VIS Protease Activity Measurement (Substrate: Casein)
Anson and Folin & Ciocalteu method and Horikoshi method
PRINCIPLE
The appearance of peptides is measured as tyrosine equivalent at 660 nm by spectrophotometry.
| Mechanism | - |
| Appearance | - |
| Longevity | - |
| Strength | - |
| Storage | - |
| Shelf Life | - |
| Allergen(s) | - |
| Dosage (Range) | - |
| Recommended Dosage | - |
| Dosage (Per Day) | - |
| Recommended Dosage (Per Day) | - |
| Mix Method | - |
| Heat Resistance | - |
| Stable in pH range | - |
| Solubility | - |
| Product Types | - |
| INCI | - |
Service Steps
| Step | Procedure | Expected Result |
|---|---|---|
| 1 | Prepare 0.1... | A clear 0.1 N NaOH solution. |
| 2 | Prepare 1... | A clear 1 N NaOH solution. |
| 3 | Prepare 0.1... | A clear 0.1 M Borax solution. |
| 4 | Prepare 0.6%... | A stable, 0.6% casein solution at pH 11.0. |
| 5 | Prepare TCA... | A clear TCA mixture solution. |
| 6 | Prepare 0.5... | A clear 0.5 M Na2CO3 solution. |
| 7 | Prepare 1... | A ready-to-use Folin�s phenol reagent at 1 N concentration. |
| 8 | Add 3.0... | Test mixture containing casein and enzyme ready for reaction. |
| 9 | Add 3.2... | Reaction stopped with visible precipitate. |
| 10 | Centrifuge for... | Clear supernatant obtained for further analysis. |
| 11 | Transfer 2... | Test sample supernatant ready for color development. |
| 12 | Add exactly... | Slightly alkaline solution ready for Folin�s reagent reaction. |
| 13 | Immediately add... | Formation of a blue-colored complex with peptides containing tyrosine. |
| 14 | Incubate this... | A stable blue-colored solution proportional to proteolytic activity. |
| 15 | Prepare a... | A blank solution for absorbance correction. |
| 16 | After incubation,... | Absorbance readings at 660 nm for test and blank samples. |
| 17 | Prepare a... | Standard curve generated for protease enzyme activity determination. |
| 18 | Use the... | Enzyme activity data recorded in units/mL. |
| 19 | Record the... | Absorbance readings of test and blank samples recorded. |
| 20 | Subtract the... | Net absorbance calculated for the test sample. |
| 21 | Use the... | Final enzyme activity reported in units/mL. |
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